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Binding of Biotin to Streptavidin: A combined fluorescence correlation spectroscopy and time-resolved fluorescence study

Strömqvist, J. ; Nardo, L. ; Broekmans, a, O. ; Kohn, Jos ; Lamperti, M. ; Santamato, A. ; Shalaby, M. ; Sharma, G. ; Di Trapani, P. ; Bondani, M. ; Rigler, R.

In: The European Physical Journal - Special Topics, 2011, vol. 199, no. 1, p. 181-194

The Biotin-Streptavidin complex is a widely studied system in biology and biophysics, because of its extremely strong non-covalent binding affinity. The latter is often exploited to link molecules to substrates or to one another. However, the details of the Biotin-Streptavidin binding have not been fully elucidated so far. Particularly, the role of cooperative effects in enhancing the binding...